Micropipettes
Solution preparation relies upon the precise measurement of volumes to obtain the exact concentrations required. Therefore, the choice of micropipette that is used can determine not only the accuracy, but also the reproducibility of your experiments.
At Ossila, we offer a range of micropipette volumes from 1 μl all the way up to 10 ml. Each micropipette comes pre-calibrated and tested to ensure accuracy and reproducibility. Additionally, we have prepared several handy guides on how to use your micropipette to get the best results possible.
Jump to: Browse micropipettes | Choose a micropipette | Tips for using a micropipette | Resources and support
Browse Micropipettes and Accessories
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Choosing a Micropipette
| Volume Range | 1 – 10 μl | 10 – 100 μl | 100 – 1000 μl | 1 – 10 ml |
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| Available in the Micropipette Set |  |
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| Accuracy | ±2.5 – 1.0% | ±1.8 – 0.8% | ±1.5 – 0.6% | ±2.5 – 0.6% |
| Repeatability | ≤1.5 – 0.4% | ≤0.5 – 0.15% | ≤0.3 – 0.15% | ≤0.3 – 0.15% |
| Includes Compatible Tips | |
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Tips for Using Your Micropipette
Using your micropipette correctly ensures accurate and reproducible dispensing in your experiements.
Withdrawing
- Do not fully insert the tip, immerse it around 1 cm into the solution. This prevents you from taking air into the micropipette.
- For viscous solutions, release the plunger very slowly to ensure that no air bubbles form in the tip.
- Keep the micropipette facing downwards while there is solution in the tip. This stops the solution from running into the piston mechanism, which will damage and contaminate it.
Dispensing
- Select an appropriate disposable tip that matches the volume to be dispensed and ensure it is securely attached.
- Release the plunger slowly and steadily to ensure accurate volume delivery.
- Dispense the liquid close to the vessel wall to prevent splashing but avoid touching the sides of the container or vessel to prevent contamination.
- Small volumes may need you to dispense directly into the solution by immersing the tip, or dispense onto the side of the vial.
- Replace the tip after each dispense to avoid cross contamination.
Calibration and Maintenance
- Recalibrate micropipettes if they are set to a volume outside of its stated operational range to ensure that you are still getting the correct volumes.
- If the micropipette is dropped, you may need to recalibrate it.
- Before use, visually inspect the micropipette for any damage or contamination and ensure that it is clean and in good working condition.
- After use, eject the tip using the tip ejector mechanism. Ensure that the tip is ejected into a suitable waste container to prevent contamination.
Resources and Support
Getting Started with a Micropipette
Using your micropipette correctly ensures the most accurate and reproducible measuring and dispensing of solutions.
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Micropipette: Positioning and Withdrawing
Follow our guide to correctly position and withdraw liquid with your micropipette.
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Micropipettes: Components and Parts
Micropipettes are indispensable tools in laboratories. These can be used in many applications ranging from thin film deposition by spin coating to cutting-edge molecular biology experiments like PCR (Polymerase Chain Reaction).
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Dispensing Liquid from Micropipette
Micropipettes allow liquids to be measured and dispensed both precisely and quickly. Therefore, micropipettes are used in a wide range of applications from microbiology to analytical chemistry.
Read more...